CD47 Mouse mAb (APC) - Primärer Antikörper, specific to CD47, Mouse IgG1

Cat. No.: Ab185154

GRADE & PURITY ExactAb™, Validated, Ex:650nm, Em:660nm, 1 μL/test

Application

Flow Cytometry

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

CC2C6

Conjugation

APC

Übersicht

Mouse anti Human CD47 Antibody, Monoclonal (CC2C6), could be used for Flow and so on.

Specifications

Product Name

CD47 Mouse mAb (APC) - Primärer Antikörper, specific to CD47, Mouse IgG1

Synonyme

antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | IAP | IAPintegrin associated protein |

Spezifikationen & Reinheit

ExactAb™, Validated, Ex:650nm, Em:660nm, 1 μL/test

Biochemische und physiologische Mechanismen

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, bind

Wirtsspezies

Maus

Spezifität

CD47

Immunogen

CCRF-CEM T-cell line.

Positive Kontrolle

Flow: MCF-7

Konjugation

APC

Anregung (nm)

Red Laser(633nm)

Emission (nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

Note

Em:660nm, Ex:650nm, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

1 μL/test

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD47 Mouse mAb (APC) (Ab185154)

CD47 Mouse mAb (APC) (Ab185154) - Flow Cytometry
Flow cytometry analysis of CD47 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with CD47 Mouse mAb (APC) (Ab185154) at 1μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

Flow Cytometry

1 μL/test, the suggested use of this reagent is 1 µL per million cells in 100 µL staining volume or 1 µL per 100 µL of whole blood

Zugehörige Ziele (menschlich)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD47 Mouse mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.