Letaplimab (anti-CD47) - Primärer Antikörper, specific to CD47, Human IgG4SP

CAS: 2283356-07-8 Cat. No.: Ab190155

GRADE & PURITY Animal Free, Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Mensch

Reactive species

Cynomolgus-Affe, Mensch

Isotype

Human IgG4SP

Conjugation

Unconjugated

Übersicht

Letaplimab (anti-CD47) is a human IgG4SP anti-CD47 monoclonal antibody. CD47 is overexpressed on cancer cells, it is a ligand for SIRPα. Letaplimab (anti-CD47) enhances tumor cell phagocytosis.
Purity
≥95% (SDS-PAGE&SEC)
Endotoxin Level
≤1.0EU/mg
Protein Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Specifications

Product Name

Letaplimab (anti-CD47) - Primärer Antikörper, specific to CD47, Human IgG4SP

Synonyme

Antigen identified by monoclonal antibody 1D8 antibody | Antigenic surface determinant protein OA3 antibody | CD 47 antibody | CD47 antibody | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody | CD47 antigen antibody | CD47

Spezifikationen & Reinheit

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Wirtsspezies

Mensch

Spezifität

CD47

Konjugation

Unconjugated

Note

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Isotyp

Human IgG4SP

Lichterketten-Typ

kappa

SDS-PAGE

142.38 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

CAS

2283356-07-8

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Flow Cytometry: Letaplimab (anti-CD47) (Ab190155)

Letaplimab (anti-CD47) (Ab190155) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD47 (red) with Letaplimab (anti-CD47) (Ab190155) at 1.0 μg/mL for 1 hour at 4°C. Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

ELISA: Letaplimab (anti-CD47) (Ab190155)

Letaplimab (anti-CD47) (Ab190155) - ELISA
Immobilized Recombinant Human CD47 protein (rp144028) at 1.0 μg/mL can bind Letaplimab (anti-CD47) (Ab190155) with the EC ₅₀  of 182.6 ng/mL.

SEC: Letaplimab (anti-CD47) (Ab190155)

Letaplimab (anti-CD47) (Ab190155) - SEC
The purity of Letaplimab (anti-CD47) (Ab190155) is more than 95% verified by HPLC.

Zugehörige Ziele (menschlich)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2283356-07-8.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Letaplimab (anti-CD47)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.