Carrier Free,Low Endotoxin,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE&HPLC),See COA Azide Free,Carrier Free,Low Endotoxin,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Übersicht

Mouse anti Human CD8 alpha Antibody, Monoclonal (UCHT4), could be used for Flow, WB, IP, IHC, IF, ICC, ELISA, Functional Assays and so on.
Application
Flow: ≤ 1.0µg/100w cells
WB: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Functional Assays: Use at an assay dependent concentration.

Protein Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Specifications

Product Name

CD8 alpha Mouse mAb - Primärer Antikörper, specific to CD8 alpha, Mouse IgG2a

Synonyme

p32 | Leu2 | IMD116 | CD8alpha | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-c

Spezifikationen & Reinheit

Carrier Free,Low Endotoxin,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE&HPLC),See COA

Wirtsspezies

Maus

Spezifität

CD8 alpha

Positive Kontrolle

Flow Cyt: mouse peripheral lymph node

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, Low Endotoxin, PBS Only, Validated

Isotyp

Mouse IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Flow Cytometry: CD8 alpha Mouse mAb (Ab095360)

CD8 alpha Mouse mAb (Ab095360) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 alpha with CD8 alpha Mouse mAb (Ab095360) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD8 alpha Mouse mAb (Ab095360)

CD8 alpha Mouse mAb (Ab095360) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 alpha (red) with CD8 alpha Mouse mAb (Ab095360) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD8 alpha Mouse mAb (Ab095360)

CD8 alpha Mouse mAb (Ab095360) - Flow Cytometry
Flow Cytometry analysis of MOLT-4 cells labelling CD8 alpha (red) with CD8 alpha Mouse mAb (Ab095360) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Dilution info

Flow Cytometry

≤1.0µg/100w cells