α-Chymotrypsin from bovine pancreas, CAS No.9004-07-3

CAS: 9004-07-3 Cat. No.: C128653 EC Number: 232-671-2 PubChem CID: 4052643

GRADE & PURITY EnzymoPure™, ≥45 units/mg protein

Accession #

P00766

Overview

Product Describtion:

Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.Three times crystallized alpha chymotrypsin which is an activation product of a 3X crystallized zymogen. Dialyzed against 1mM HCl and lyophilized.  

Specifications

Product Name

α-Chymotrypsin from bovine pancreas, CAS No.9004-07-3

Synonyms

N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate | Chymotrypsin Substrate I, Colorimetric | N-(3-Carboxy-1-oxopropyl)glycylglycyl-N-(4-nitrophenyl)-L-phenylalaninamide | AKOS024350585 | 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]ami

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥45 units/mg protein

Biochemical and Physiological Mechanisms

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide c

Accession #

P00766

CAS

9004-07-3

Enzyme Commission Number

3.4.21.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥45 units/mg protein

Storage

Store at 2-8°C

Shipped In

Wet ice

Unit definition

One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg usi

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