IHC

Step-by-step protocol for PKC-beta 1 Mouse mAb (Ab349200): recommended dilutions, sample prep, WB, IHC, and storage conditions for optimal results.

Step-by-step guidance on selecting and using IGSF3 Mouse mAb (Ab351255) for WB, IHC and ICC. Covers dilution, storage, and troubleshooting tips.

Blocking is a critical step for controlling nonspecific background in immunoassays such as Western Blot, ELISA, IHC, and IF, but the targets of blocking are not completely identical among different experiments. BSA, Casein, and protein-free blocking solutions are mainly used to limit nonspecific ...

TSA, or Tyramide Signal Amplification, is a signal amplification technique based on HRP (Horseradish Peroxidase)-catalyzed reactions. It is commonly used in IHC (Immunohistochemistry), ICC (Immunocytochemistry), ISH (In Situ Hybridization), FISH (Fluorescence In Situ Hybridization), and ...

The washing step in IHC directly affects two types of outcomes. One is whether unbound or weakly bound detection reagents can be sufficiently removed. The other is whether specifically formed binding complexes can be stably retained.

Antigen retrieval in IHC determines the extent to which epitopes masked after fixation can be re-exposed, and it directly affects localization quality, signal intensity, and tissue integrity.

Immunohistochemistry Protocol

Immunohistochemistry (IHC) is a technique that uses the principle of specific binding between antigens and antibodies to locate, characterize and quantify antigens (peptides and proteins) within tissue cells by chemically reacting the marker antibody's color developer (fluorescein, enzyme, metal ...

IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate?

How many slices can I do with 20μI when I have IHC lab-applicable antibodies?

What does IHC mean on the data sheet? Is it frozen section? Is it different from IHC-F?

Automated Immunohistochemical Staining Protocol for Anti-PD-L1

Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to ...

Immunohistochemical staining protocol for frozen samples (IHC-Fr)

Whole mount staining is the staining of small pieces of tissue-usually embryos-without sectioning. 

Antibodies are powerful research tools used in a variety of laboratory techniques. Here, we will provide a brief overview of the most popular laboratory techniques, focusing on how they use antibodies.