EGFR Mouse mAb (AF405) - Primary antibody, specific to EGFR

Cat. No.: Ab217688
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 5 μL/test
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG2b
Clone Number
EGFR.1
Conjugation
AF405
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10T
Ab217688-10T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$179.90
25T
Ab217688-25T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$347.90
100T
Ab217688-100T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$939.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 5 μL/test ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human EGFR Antibody, Monoclonal (EGFR.1), could be used for Flow Cytometry.

Specifications

Product Name
EGFR Mouse mAb (AF405) - Primary antibody, specific to EGFR
Synonyms
Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody | Cell growth inhibiting protein 40 antibody | Cell proliferation inducing protein 61 antibody | EGF R antibody | EGFR antibody | EGFR_HUMAN antibody | Epidermal growth factor recepto
Specifications & Purity
ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,
Host species
Mouse
Specificity
EGFR
Immunogen
A431 cultured cells
Positive Control
Flow Cytometry: A431
Conjugation
AF405
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

EGFR Mouse mAb (AF405) (Ab217688) - Flow Cytometry
Flow cytometry analysis of EGFR expression by A431 cells (red) compared to an isotype control (blue). Cells were stained with EGFR Mouse mAb (AF405) (Ab217688) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
EGFR Tclin Epidermal growth factor receptor (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0521640Certificate of AnalysisMar 16, 2026 Ab217688
ZJ25F0521641Certificate of AnalysisMar 16, 2026 Ab217688
ZJ25F0521642Certificate of AnalysisMar 16, 2026 Ab217688
Solution Calculators
Reviews

Customer Reviews

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Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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