Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Übersicht

Mouse anti Human EGFR Antibody, Monoclonal (528), could be used for in vitro EGFR blockade, in vivo EGFR blockade in xenografts, WB, Functional assays, IP, IHC, ICC, IF, Flow and so on.

Application:

in vitro EGFR blockade: Use at an assay dependent concentration.

in vivo EGFR blockade in xenografts: Use at an assay dependent concentration.

WB: Use at an assay dependent concentration.

Functional assays: Use at an assay dependent concentration.

IP: Use at an assay dependent concentration.

IHC: Use at an assay dependent concentration.

ICC/IF: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

Specifications

Product Name

EGFR Mouse mAb - Primärer Antikörper, specific to EGFR, Mouse IgG2a

Synonyme

Spezifikationen & Reinheit

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Wirtsspezies

Maus

Spezifität

EGFR

Immunogen

Purified EGFR from A431 cells

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotyp

Mouse IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Bilder

Flow Cytometry: EGFR Mouse mAb (Ab101169)

EGFR Mouse mAb (Ab101169) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with EGFR Mouse mAb (Ab101169) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Dilution info