GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human EGFR Antibody, Monoclonal(C4), could be used for WB, IHC and so on.
Specifications
Product Name
EGFR Mouse mAb - Primary antibody, specific to EGFR, IgG2b
Carrier Free, ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,
Host species
Mouse
Specificity
EGFR
Immunogen
Recombinant EGFR expressed in E.coli (AA 888-1210)
Positive Control
WB: A431, HeLa, MCF7, A549, NIH/3T3 and HUVEC cell lysates. IHC: Human kidney, placent, lung cancer, stomach, adrenal gland, liver cancer tissues. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
IgG2b
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
EGFR Mouse mAb (Ab101163) - Western Blot All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 175 kDa Observed band size: 169 & 69 kDa Exposure time: 10.4 s
EGFR Mouse mAb (Ab101163) - Western Blot All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 175 kDa Observed band size: 169 & 69 kDa Exposure time: 2.3 s
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Placenta Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Lung cancer Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Stomach Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Adrenal gland Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Liver cancer Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IF/ICC IF analysis of EGFR Mouse mAb (Ab101163) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with EGFR Mouse mAb (Ab101163) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
5 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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