Hemoglobin and red blood cell count is mildly to moderately reduced, some cases of severe reduction, white blood cell count in most cases below 15 × 109 / L, but there are also normal or significantly higher or lower. Decrease can be manifested as a decrease in whole blood cells, classification of abnormal early granulocytes are predominant, can be as high as 90% of the visible few granulocytes and other stages of granulocytes Auer microsomes are easy to see. Moderate to severe thrombocytopenia .....
This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory instruction.
Operation method
Acute promyelocytic leukemia detection test Move Blood: hemoglobin and red blood cell count is mild to moderate reduction, some cases of severe reduction, white blood cell count in most cases below 15 × 109 / L, but there are normal or significantly higher or lower. Decrease can be manifested as a decrease in whole blood cells, classification of abnormal early granulocytes, up to 90% of the visible minority of granulocytes and other stages of granulocytes Auer microsomes easy to see. Platelets are moderately to severely reduced, mostly (10-3O)×1012/L. The platelet counts are usually low. For more product details, please visit Aladdin Scientific website.
Bone marrow image: most cases of bone marrow hyperplasia is extremely active, individual cases of hyperplasia, classification of granules with more early granulocytes, accounting for 30-90% (NEC) can see a certain number of progranulocytes and medium-granulocytes, early granulocytes and primitive granulocytes ratio of 3:1 or more, the stages of young erythrocytes and megakaryocytes are significantly reduced, the granularity increased in early granulocytes. The morphology of granulocytes is abnormal, and the size of such cells varies, generally 15-3O micrometers in diameter. The shape is mostly irregular, round, round-like kidney-shaped. Malformations. Depressed, folded, twisted or lobulated, the nucleus is slightly small and often off-set, some can be seen double nuclei, nucleoli l-3, some are covered by particles and unclear, cytoplasmic abundance of blue or gray cytoplasm containing a large number of varying sizes of aniline blue particles, purplish-red and dense, more distributed in the cytoplasm at one end of the nucleus, around the nucleus or cover the nucleus, some cytoplasm can be divided into inner and outer layers, in the cell Some cytoplasm can be divided into two layers, the outer cytoplasmic layer at the edge of the cell particles sparse or no particles, some cytoplasm contains short and thick Auer's vesicles, a few, a dozen or dozens of them, can be bundled cross, arranged, resembling faggot-like, so some people call it "faggot cell" (faggot cell) according to different particles are also divided into two subtypes. The faggot cell is divided into two subtypes according to the different particles:
1, coarse granular type ( M3a ) cytoplasm rich, blue outer cytoplasm was pseudopod-like protruding which is full of coarse, deeply stained, dense or fusion of aniline blue particles, or containing more Auer's vesicles, and sometimes in the form of "faggot", and the nucleus of the cell is often covered by the particles and the contour of the outline is not clear.
2, fine granular type ( M3b ), the cytoplasm of the aniline blue particles dense and small nuclei are twisted and folded or lobulated, so it is easy to be misdiagnosed as a mononuclear fine, part of the patient's early granulocyte cytoplasm is strongly alkaline, granules are sparse, the nucleus is clearly lobulated. Cytochemical staining: POX, SB, AS-D-NCE and ACP staining were positive or strong positive reaction, AS-D-NAE can be negative, not only not inhibited by sodium fluoride, a-naphthol butyrate esterase staining was positive, according to this can be identified with acute single.