β-Propiolactone (BPL) Viral Inactivation — Standard Operating Procedure (SOP)
β-Propiolactone (BPL) Viral Inactivation — Standard Operating Procedure (SOP)
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Standard Operating Procedure for Adipogenic Differentiation of Mesenchymal Stem Cells and Evaluation by Oil Red O Staining
This procedure is intended to establish an in vitro adipogenic induction and Oil Red O staining evaluation workflow for mesenchymal stem cells (MSCs). It is applicable to adipogenic differentiation studies using MSCs derived from bone marrow, adipose tissue, umbilical cord, and other sources.
Experimental purification of λ phage particles by precipitation/centrifugation
If the purpose of purification of the phage pellet is to obtain outgrown DNA fragments for subsequent subcloning or to prepare phage two-arms, centrifugation ca
Standard Operating Procedure for Chondrogenic Differentiation of Mesenchymal Stem Cells and Evaluation by Alcian Blue Staining
This procedure is intended to establish an in vitro chondrogenic pellet induction and Alcian Blue staining evaluation workflow for mesenchymal stem cells (MSCs). It is applicable to chondrogenic differentiation studies using MSCs derived from bone marrow, adipose tissue, umbilical cord, and ...
Experimental preparation of λ phage particles from large-scale lysates
λ phage prepared from mass cultures can be recovered from the lysate by polyethylene glycolysis (PEG) precipitation in the presence of high salt. The residual
Detection of α-, β- and γ-interferon-induced antiviral activity
The cells used in the assay are mouse L 929 fibroblasts, which have a high sensitivity to interferon and V S V . The cells should be derived from laboratories t
Standard Operating Procedure for Osteogenic Differentiation of Mesenchymal Stem Cells and Evaluation by ALP and Alizarin Red S Staining
This procedure is intended to establish an in vitro osteogenic induction and differentiation evaluation workflow for mesenchymal stem cells (MSCs). It is applicable to osteogenic differentiation studies using MSCs derived from bone marrow, adipose tissue, umbilical cord, and other sources. The ...
Standard Operating Procedure for Frozen Tissue Section Preparation and Section Storage
This procedure is intended to standardize tissue pretreatment, fixation, sucrose cryoprotection, OCT embedding, rapid freezing, cryosectioning, section collection, frozen storage, and thawing procedures. It is applicable to immunofluorescence, immunohistochemistry, enzyme histochemistry, lipid ...
Helicobacter pylori May-Grünwald-Giemsa (MGG) Staining Protocol
This protocol is used to establish a routine procedure for Helicobacter pylori May-Grünwald-Giemsa (MGG) staining. It is suitable for the morphological visualization of Helicobacter pylori in paraffin-embedded gastric mucosal tissue sections and can be used for auxiliary observation and ...