Blood sampling from rat and mouse large blood vessels

Summary

This experiment is mainly used to draw blood from rats and mice.

Operation method

Blood sampling from rat and mouse large blood vessels

Principle

Blood can be taken from the jugular artery, femoral artery and abdominal aorta. In these parts of the blood extraction must be anesthetized and fixed animals, and then make the arterial (static) vein separation surgery, so that it is fully exposed, with a syringe along the parallel direction of the large blood vessels to stab, to extract the required amount of blood. Or directly use scissors to cut the large blood vessels to absorb, but when cutting off the arteries, to prevent blood spatter.

Materials and Instruments

Rats Mice
Small animal bench Syringes Alcohol

Move

1、Cutting the tail for blood collection: this method is commonly used when the amount of blood required is small. First, the animal will be fixed, the rat tail immersed in warm water at about 50 ℃ for a few minutes or with alcohol cotton balls or xylene rubbing the rat tail, so that the tail blood vessels are full, cut off the tail tip 1 ~ 2mm (mice) or 3 ~ 5mm (rats), then the blood will be free to flow along the blood vessel wall into the test tube or hemoglobin pipette to absorb. At the end of blood collection, the wound is sterilized and the bleeding is stopped by cotton ball pressure. This method can generally be more than 10 times per rat, mice can take about 0.1mL of blood each time, rats can take 0.3-0.5ml of blood.

2. Post-orbital venous plexus blood collection: the operator fixes the mouse or rat with one hand, and pinches the skin of the mouse head as tightly as possible with the thumb and forefinger, or gently presses both sides of the neck, so as to make the mouse's eyeball protrude and the post-orbital venous plexus congested, and then holds a capillary blood collection vessel with the other hand to pierce the blood collection vessel at 45° from the corner of the inner eye and rotate downward, and then back outward while suctioning after the feeling of stabbing the venous plexus, when getting the required amount of blood, relaxing the pressure on the neck, and pulling out the blood collection device to prevent the blood collection. When the desired amount of blood is obtained, the pressure on the neck is relaxed and the blood collector is withdrawn to prevent bleeding from the puncture hole. If the technique is skillful, this method can be repeated to collect blood in a short period of time, mice can collect 0.2-0.3 ml of blood at one time, and rats can collect about 0.5 ml of blood. if only one blood collection is carried out, the method of removing the eyeball can be used, the right hand takes the curved forceps of Ophthalmology, and removes the eyeball from the root of the eyeball of the rat's right or left eyeball, and the rat is inverted; the head is turned downward, and the blood is extracted.

3、Mouse loaded into the fixator, leak out the tail, the same 1, so that the tail blood vessels are filled, make a good tail disinfection, with a 1 ml syringe connected to the No. 4 needle, puncture the tail vein, extract blood.

4、Axillary blood collection: fix the anesthetized mice in supine position, cut open the axillary skin, bluntly separate the pectoral muscles and other tissues in the axilla, expose the axillary blood vessels, cut the axillary vein, and draw blood with a syringe or pipette.

5、Blood collection from severed head: use scissors to quickly cut off the animal's head, immediately turn the animal's head down, lift the animal, and the blood can flow into the test tube.

6、Blood collection from the heart:Cut open the animal's chest and collect blood directly from the heart, or cut the heart and suck the blood directly with a syringe or pipette.

7、Femoral artery blood collection:This method is commonly used when a large amount of blood is taken. Need to surgically separate the femoral artery, use a syringe with a needle to pierce the blood vessel to collect blood or cut the femoral artery with a pipette to absorb blood samples, the amount of blood collected at one time can be up to 0.5 ml in mice and up to 2.0 ml in rats, and to prevent the spraying of blood during the operation.

Caveat

1. prevent lint from entering the bloodstream causing hemolysis, etc.

2. to prevent violent shaking, generally after collection must be left to stand and centrifuged to collect serum (or plasma).

3. Do not freeze before separation. The serum (plasma) after separation may be frozen as appropriate.

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