Cerebrospinal fluid microscopy experiment
Cerebrospinal fluid microscopy experiment
Cerebrospinal fluid (CSF) microscopy is used to count and categorize cerebrospinal fluid (CSF) cells to provide a basis for determining various diseases. This experiment is from the Mudanjiang Medical College undergraduate 5-year clinical laboratory guide.
Operation method
Cerebrospinal fluid microscopy experiment Move First, the total number of cells Caveat csf collection should be counted within 1 hour, such as shelved for too long cell destruction or precipitation of fibrous Pr clots, can lead to inaccurate counting.(1) puncture damage to blood vessels resulting in bloody csf at this time the total number of cell count is meaningless, do the correction is as follows: will be mixed csf diluted with 1% glacial acetic acid for the discharge of bloody csf RBC impact, the correction is as follows:Total number of corrected csfWBC = total number of uncorrected scfWBC - (RBCs in csf x WBCs in surrounding / number of RBCs in surrounding blood).(2) During cell counting, if more RBCs are found to be wrinkled and swollen, they should be reported.(3) When counting cells, it should be noted that the LC on the RBC is different from the new cryptococcus.(4) The fixation time of the smear should not be too long. For more product details, please visit Aladdin Scientific website.
1, direct counting method csf specimen observation, clear or only slightly mixed, can be used in this method with a dropper has been shaken csf specimen a small amount of direct filling into the counting chamber of the counting plate, static for 2-3 minutes under the low magnification microscope to count the number of cells in the corners of two counting pools and the center of a total of 10 cell number, i.e., the total number of cells of the 1 μl of csf.
2, dilute the counting method such as too much specimen csf, can be used to dilute saline and RBC diluent according to the above method of counting multiplied by the dilution times. If there are too many cells in the specimen, use saline and RBC diluent to dilute it according to the above method, and multiply the result by the dilution times.
Second, white blood cell counting
1, direct counting method: use a pipette to suck all the ice acetic acid after blowing out, so that the tube avoided attached to a small amount of ice acetic acid, and then use the same pipette to suck a small amount of mixed csf specimen, a few minutes after the dissolution of RBC, and then filled with the counting chamber, according to the method of counting.
2, such as the WBC is too much, with WBC diluent dilution, multiply the results by the number of dilutions. Count according to the above method, and multiply the result by the number of times of dilution.
Third, WBC classification and counting:
l, direct classification method; WBC direct counting, turn high power microscope observation, according to the morphology of the cell and the morphology of the nucleus, a total of 100 WBC were calculated single nucleated cells and multinucleated cell ratio, expressed as a fraction of, if the WBC is less than 100 can be written directly single nucleated cells and multinucleated cell number, if the total number of WBC in the 30 or less, can not be done classification.
2, staining classification method centrifugation of csf specimen, take the precipitate smear, make a uniform film. Place the specimen at room temperature or 37℃ in a warm box, dry and Wright staining, and count under the oil microscope.