Experiment on isolation of lactic acid bacteria in kimchi

Summary

Lactic acid bacteria are some partially anaerobic, Gram-positive, rod-shaped or globular, non-spore, non-motile, poor decomposition and synthesis ability, high nutritional requirements, need to provide a rich supply of peptides, amino acids and vitamins, they lack respiratory chain components, superoxide dismutase and catalase, and in the surface of the agar medium or the inner layer of the agar medium only form smaller white or light-colored colonies. The present experiment was carried out to isolate lactic acid bacteria of interest in kimchi on calcium carbonate agar medium of tomato juice using smear plate method.

Operation method

coated plate method

Principle

Lactobacilli are some partially anaerobic bacteria, Gram-positive, rod-shaped or globular, no spores, non-motile, poor decomposition and synthesis ability, high nutritional requirements, the need to provide a rich supply of peptides, amino acids and vitamins, they lack respiratory chain components, superoxide dismutase and catalase, on the surface of the agar medium or the inner layer of the agar medium to form only a smaller white or light-colored colonies. Commonly used isolation media are tomato juice calcium carbonate agar medium, malt juice calcium carbonate agar medium. The isolation methods are plate streaking method or coated plate method after appropriate dilution.

Materials and Instruments

Tomato Juice Calcium Carbonate Agar Medium Pickle Juice
Glucose Yeast Paste KH2PO4 Tween Agar Tomato Juice Water
Petri dishes Straws Mantles Inoculation rings Triangular flasks Slides

Move

I. Preparation of tomato juice calcium carbonate agar medium
Pour flat dish and set aside when solidified;II. Plate separation
Single colonies were isolated from good quality kimchi juice by direct plate streaking or by smear plate method after appropriate dilution;III. Constant temperature culture
The above plates were put into 25 ℃ and 37 ℃ incubator for more than 48 h, and could also be put into anaerobic tank for incubation;IV. Observation of colonies
Lactobacilli form only small light-colored (usually yellowish or white) colonies on the surface of the above plate medium;V. Preservation of transplanted seeds
The colonies were inserted into slant test tubes and incubated for preservation purposes.

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