Protocols

Extraction and activity of ATPase from cardiac myofibrillar fibers

Summary

Cardiac myofibrillar ATPase is Ca2+, Mg2+ ATPase, which breaks down ATP into ADP and Pi and releases energy to provide myofibrillar contraction needs. Myofibrils were extracted by Salaro and Pagani method, mainly by differential centrifugation and Triton X-100 stripping. The enzyme activity was determined in much the same way as that of the myoplasmic reticulum Ca2+ATPase.


For more product details, please visit Aladdin Scientific website.

https://www.aladdinsci.com/

Categories: Protocols
Explore topics: protein experiment

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "Extraction and activity of ATPase from cardiac myofibrillar fibers" Aladdin Knowledge Base, updated Dec 23, 2024. https://www.aladdinsci.com/us_en/faqs/extraction-and-activity-of-atpase-from-c-en.html
Was this article helpful? Yes No 0 out found this helpful

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.