Handling of Petri dishes and plates

Summary

The manipulation of Petri plates is necessary for cell experiments such as (1) cell growth (2) cell propagation (3) cell inoculation.

Operation method

Handling of Petri dishes and plates

Principle

A Petri dish is a laboratory vessel used for the culture of microorganisms or cells, consisting of a flat disc-shaped base and a lid, usually made of glass or plastic. It was first designed in 1887 by bacteriologist Julius Richard Petri (Julius Richard Petri, 1852-1921), who worked under German biologist Robert Koch, so it is also known as "Petri dish". Petri dishes are fragile and brittle, so they should be handled with care and placed gently during cleaning and handling. After the use of petri dishes zui good timely cleaning, stored in a safe, fixed position, to prevent damage, broken.

Materials and Instruments

Petri dishes Petri plates

Move

Removing Media

1. Place the petri dish or plate to the side of the workbench.

2. Turn on the suction pump.

3. Pick out the uncottonized straw and poke it into the suction tube of the suction pump.

4. Place the petri dish or plate in the center of the workspace.

5. Remove the lid of the petri dish or plate and place it, mouth-side up, at the back of the dish or plate.

6. Pick up the dish as close to the bottom as possible. Be careful not to touch the edges of the petri dish and not to pass your hand over the open end.

The computer generates the optional text: Petri dish or its lid (with practice, it is possible to open the lid well without removing the lid completely and to tilt the dish to facilitate the removal of the old medium, which is quicker and safer than the method described earlier).

7. Tilt the dish to remove the medium. If no pump is available, discard the used medium into the waste beaker.

8. Replace the lid.

9. Move the Petri dish to the work area and place it in the same place as the other Petri dishes. Move the petri dish to the side of the work area opposite the untreated petri dishes.

10. Do the same with the rest of the petri dishes and plates.

11. Dispose of the pipette and turn off the suction pump.

Adding Medium or Cells

1. Place the necessary reagent bottles in place and loosen the lids of the bottles you will be using.

2. Place the Petri dish in the center of the work area.

3. Remove the lid and use the pipette to aspirate from the reagent bottles.

4. Place the lid on the back of the Petri dish.

5. Gently inject the medium into the bottom of the base of the Petri dish by gently pushing on the Petri dish on one side of the Petri dish.

6. Close the lid of the Petri dish.

7. Put the Petri dish on its original side. Place the Petri dish on its original side. Be careful not to allow the medium to enter the small space between the lid and the bottom.

8. Follow the same procedure for the second and remaining petri dishes.

9. Discard the pipette.

Again, with practice, you will be able to add medium without removing the lid, just as you did with the medium.

Caveat

Petri dishes and multi-well plates are particularly susceptible to contamination for the following reasons.1. The exposed surface area of the petri dish opening is large.

2. The risk of touching the edges of the petri dish when handling open petri dishes.

Common Problems

There are glass plates and glass dishes available in the market today.

1, Glass-bottomed multi-well culture plates have the same glass bottom and optical properties as glass dishes, allowing researchers to use high volume production, high throughput screening, and less precious reagents used;

2, The biggest advantage of multiwell glass bottom plates is that you can culture 6,12,24,95 well cultures on the same plate under consistent conditions. Multiwell plates are suitable for high throughput, high volume screening field. Analysis using multiwell plates is streamlined because only one plate needs to be processed (as opposed to using multiple dishes);

3, for some fields, the use of multiwell plates to handle cultures becomes simpler (eg: luminescence) multiwell plates have smaller pore sizes, which is extremely effective for small volumes of valuable reagents;

4, glass-bottomed dishes have the dual characteristics of both flat dishes and coverslips:

① the bottom of the coverslip for the excellent light transmittance of optical glass, light transmittance is better than plastic dishes;

② the thickness of the bottom coverslip is not greater than 0.17mm, suitable for high-magnification observation and high-quality imaging;

③ observation. Cells covered with liquid above, do not have to add a coverslip, so it can keep the cells and other samples are not deformed, not dry, to maintain its original form;

④ Liquid can be added or sucked out of the dish at any time, so the cells can be processed at any time such as washing or adding stimulants, which is conducive to maintaining the growth of cells and experimental operations;

⑤ When observing the cells with an inverted microscope, it can be observed with the lid of the glass-bottomed dish, which maintains the sterility of the cells and facilitates cell culture;

⑥ Save reagents and cells, simplify the operation process.

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