Morphological observation experiments on bone abrasions
Morphological observation experiments on bone abrasions
Morphologic observation experiments on bone grindings can be applied to (1) examine the number of intracortical bone units, the number of old bone units (2) examine the thickness of the external ring bone plate, and the number of non-Harvard's canals, etc., and (3) discuss the determination of the species and age of the skeleton.
Operation method
Morphological observation experiments on bone abrasions
Principle
Bone is hard connective tissue. Under the microscope to observe the bone tissue structure of the bone grinding piece, it is necessary to make the bone tubules and the bone fossa filled with air and become black, so that when observed under the microscope, it can be clearly visible, so the sealing piece is one of the difficulties in the production of bone grinding piece technology. Generally, liquid gum can not be used to seal the bone grinding film, because xylene in the gum will make the bone tubules and the bone fossa transparent, and the structure will not be clear. In order to better show the structural integrity of bone tissue, and make the grinding film is not easy to fall off. The production of good bone and dental grinding plate, for the teaching of tissue embryo is very necessary. Initially, thin slices of sawn bone are placed on a whetstone or glass plate and wet ground with an abrasive, such as very fine emery or household stain remover. They can also be placed between two plates of burr glass or thin bone fragments can be placed under a rubber or cork. Bone grinding sheet is the micro morphology of bone tissue, such as the shape and size of the Haver's canal, the number of levels of bone plates and bone units and other morphological features need to be examined with an optical microscope, so it is necessary to make the bone into a thin sheet that can transmit light and observe the internal structure. The method of production is to saw off a 1.5~2.0mm thick piece of bone in cross-section, and then thin it uniformly with a fine grinding stone until the bone piece is of newsprint-like thickness. Then after a series of dehydration, transparent, fixed on a slide, with a microscope to observe the desired morphological characteristics.
Materials and Instruments
Bone Grinding Pads Move 1. Coloring: silver plating Caveat Bone grinders are thicker than slices and are not uniform in thickness, so the thinner area should be selected for observation. Common Problems This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine. For more product details, please visit Aladdin Scientific website.
Silver Stain
Microscope
2. Visualization by the naked eye:
Bone grindings are thicker than slices and are not uniform in thickness; the thinner part should be selected for observation.
3. Low magnification observation:
Because the specimen is made from dry bone, there is no soft tissue in the section, and the four bone plates in dense bone are mainly distinguished:
(1) Outer ring bone plate: located on the surface of the bone; the plate is arranged parallel to the surface of the bone, with more layers and neatness.
(2) Inner ring bone plate: located in the bone marrow cavity surface, arranged along the bone marrow cavity surface, with few layers of bone plate and different thickness.
(3) Haversian system: located between the inner and outer ring bone plates, with a dark brown central canal in the center of the Haversian system, and many layers of Haversian bone plates arranged in concentric circles around the central canal.
(4) The interosseous wrench: located between the Haversian system, it is a bone plate of unequal size and irregular arrangement.
4. High magnification observation
(1) Bone trap: located between or within bone plates, single scattered arrangement, oval, dark brown.
(2) Bone tubules: many radial tubules protruding from the bone fossa in all directions, dark brown in color, adjacent tubules are connected with each other. In the Haversian system, the osseous tubules are connected to the central canal.