Antibody

Immunoglobulins are the central effector molecules of adaptive humoral immunity. They are produced by B cells and their terminally differentiated plasma cells and contribute to pathogen clearance, maintenance of immune homeostasis, and the development of immunopathology through a dual ...

Antibodies are structurally complex, highly heterogeneous macromolecules that are sensitive to external conditions and inherently less stable.

ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).

An isotype control antibody appears like a typical antibody but it does not attach itself to a specific target protein like the primary antibody used in the experiment.

There are a variety of antibodies available for detecting target proteins for any purpose. In order to narrow down the choice of antibodies and select the appropriate one, the following factors need to be considered.

In immunology experiments, it is often necessary to select different secondary antibodies to meet the experimental requirements. Usually, there may be several types of secondary antibodies available for a particular experiment, and the following aspects need to be considered to select the most ...

There are many kinds of Western Blot internal reference antibodies, such as β-actin, β-tubulin, GAPDH, Lamin B, etc., so how should we do the selection for our own experiments? Below is a brief introduction to the principles that should be followed in selecting internal reference antibodies.

Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.

Do I need to adjust the pH again after diluting EDTA Antigen Repair Solution?

What are the temperature requirements for eluting antibodies in an antibody removal solution? Can the same membrane be eluted more than once?

Can I still use it if I accidentally store it at room temperature for a day?

How long can I keep it at -20°C? Can it be stored for a short time at 4°C or room temperature? Can it be stored at -80°C?

Can the lysate be used without sodium aluminate?

Lysate lysate not lysed, add ripa and shake, then lysed on ice for 20 minutes and then centrifuged and got a gooey mess?

What concentration of BSA is recommended for WB experiments?

IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate?

How should I choose the secondary antibody?

Can the antibody be used in applications that are not described in the package insert?

If the species of my experimental sample is not included in the Reactivity of the antibody, is there any after-sales service available?

How many slices can I do with 20μI when I have IHC lab-applicable antibodies?

What does IHC mean on the data sheet? Is it frozen section? Is it different from IHC-F?

Whole mount staining is the staining of small pieces of tissue-usually embryos-without sectioning. 

Native ChIP (N-ChIP) Protocol

Immunocytochemistry (ICC) protocol

Will Aladdin antibodies recognize target protein in complex biological fluids such as blood or serum?

Will Aladdin antibodies recognize target proteins sold by other vendors?

Have Aladdin’s antibodies been tested in neutralization assays?

Are Aladdin’s antibodies suitable for use in ELISA and Western Blot applications?

Which isotype are Aladdin’s polyclonal antibodies?

What information should be known about the stability of your antibody products?

How are your antibodies purified?

Can you tell me what epitope your antibody binds to?

Do Aladdin’s antibody products contain any carrier proteins or other additives?

Will Aladdin antibodies work in immunohistochemistry and immunocytochemistry applications?

IgG, the key factor of Humoral immunity response, is the main immunoglobulin in blood, lymph, cerebrospinal fluid and peritoneal fluid. IgG accounts for approximately 75% of total serum immunoglobulins and is the only immunoglobulin that can penetrate the human placenta. It is primarily ...