Thermophilus Vitamin-Mineral Stock 1000X (TYE Media, Castenholz Media) (Powder)

Directions per Liter:

Dissolve 40.51g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Minerals	g/liter
Boric Acid	0.5
Calcium Chloride	6.6
Cobalt Nitrate	0.05
Copper Sulfate	0.15
Ferric Chloride	5
Magnesium Chloride	25
Manganese Sulfate	2
Nickel Chloride	0.02
Sodium Molybdate	0.025
Zinc Sulfate	0.5
Vitamins
p-Aminobenzoate	0.08
Biotin	0.01
Choline Bromide	0.05
Cyanocobalamin	0.0004
Folic Acid	0.01
myo-Inositol	0.02
Nicotinamide	0.08
Monosodium Orotic Acid	0.02
D-Pantothenic Acid•Ca	0.08
Pyridoxine Hydrochloride	0.08
Riboflavin	0.04
Spermidine	0.1
Thiamine Hydrochloride	0.1
	Total: 40.515g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.