GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Validated, 1.0 mg/mL Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human FHIT Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on. Application WB: 1/500-1/2000 IHC: 1/100 - 1/300 IF: 1/200 - 1/1000
Specifications
Product Name
FHIT Antibody - Primary antibody, specific to FHIT, Rabbit IgG
Synonyms
fragile histidine triad | AP3A hydrolase | AP3A hydrolase fragile site 3p14.2 | AP3Aase | Bis 5' adenosyl triphosphatase | Dinucleosidetriphosphatase | FRA 3B | FRA3B | Fragile histidine triad gene | Fragile histidine triad protein | Tumor suppressor prot
Specifications & Purity
Validated, 1.0 mg/mL
Host species
Rabbit
Specificity
FHIT
Immunogen
A synthetic peptide derived from human FHIT (AA 81-130).
Positive Control
WB: Mouse kidney, A549 lysates. IHC: Human breast carcinoma, kidney tissues. IF/ICC: A549 cells.
Conjugation
Unconjugated
Grade
Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
FHIT Antibody (Ab103425) - Western Blot All lanes: FHIT Antibody (Ab103425) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 19 kDa Exposure time: 10.3 s
FHIT Antibody (Ab103425) - Western Blot All lanes: FHIT Antibody (Ab103425) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 19 kDa Exposure time: 38.6 s
FHIT Antibody (Ab103425) - IF/ICC Immunofluorescence analysis of A549 cells, using FHIT Antibody (Ab103425). The picture on the right is blocked with the synthesized peptide.
FHIT Antibody (Ab103425) - IHC Immunohistochemical analysis of paraffin-embedded Human kidney. FHIT Antibody (Ab103425) was diluted at 1:100 (4℃, overnight). High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 30 min).
FHIT Antibody (Ab103425) - IHC Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FHIT Antibody (Ab103425). The picture on the right is blocked with the synthesized peptide.
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/100-1/300
IF/ICC
1/200-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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