GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.6.0 mg/mL
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for WB, ELISA, CLIA, LF, GICA and so on. WB: 1/400 - 1/2000 ELISA : 1/5000 - 1/15000 LF/ GICA : 0.5-1.0mg/ml Function IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
Goat IgG
SDS-PAGE
150kDa
Purification Method
Antigen affinity purified
Concentration
6.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) - ELISA Goat Anti-Rabbit IgG H&L Antibody (Ab138272) was tested by ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215) (3-fold started from 333 ng/mL). Goat Anti-Rabbit IgG H&L Antibody was then added at a series of different dilution ratios. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) - ELISA Cross-reactivity of Goat Anti-Rabbit IgG H&L Antibody (Ab138272) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Rabbit IgG H&L Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody. For the batch tested, Goat Anti-Rabbit IgG H&L Antibody (Ab138272) showed a cross-reactivity with Human IgG when the dilution ratio of Goat Anti-Rabbit IgG H&L Antibody is less than 1/8000.
Application
Application
Dilution info
LF/GICA
0.5-1.0 mg/mL
WB
1/400 - 1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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