GNMT Mouse mAb - Primärer Antikörper, specific to GNMT, Mouse IgG2a

Cat. No.: Ab105966

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

Maus

Reactive species

Maus, Mensch

Isotype

Mouse IgG2a

Clone Number

A41S18

Conjugation

Unconjugated

Übersicht

Mouse anti Human GNMT Antibody, Monoclonal (A41S18), could be used for WB, Flow and so on.

Specifications

Product Name

GNMT Mouse mAb - Primärer Antikörper, specific to GNMT, Mouse IgG2a

Synonyme

EC 2.1.1.20 antibody | Epididymis secretory sperm binding protein Li 182mP antibody | Glycine N methyltransferase antibody | Glycine N-methyltransferase antibody | Gnmt antibody | GNMT_HUMAN antibody | HEL S 182mP antibody | OTTHUMP00000016412 antibody |

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet a

Wirtsspezies

Maus

Spezifität

GNMT

Immunogen

Recombinant protein of human GNMT.

Positive Kontrolle

WB: Mouse liver lysates. Flow: U-2 OS cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: GNMT Mouse mAb (Ab105966)

GNMT Mouse mAb (Ab105966) - Western Blot
All lanes: GNMT Mouse mAb (Ab105966) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 31 kDa
Exposure time: 20.8 s

Flow Cytometry: GNMT Mouse mAb (Ab105966)

GNMT Mouse mAb (Ab105966) - Flow Cytometry
Flow cytometric analysis of U-2 OS cells using GNMT Mouse mAb (Ab105966). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37℃ for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37℃ for 40 min. Isotype control antibody (blue line) was used under the same condition.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/10-1/50;

Zugehörige Ziele (menschlich)

GNMT Tchem Glycine N-methyltransferase (0 Activities)

Discover Target: GNMT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GNMT Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.