Grp75 Mouse mAb - Primärer Antikörper, specific to Grp75, IgG2b

Cat. No.: Ab181865

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG2b

Clone Number

CD02/2E10

Conjugation

Unconjugated

Übersicht

Application

WB: 1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa

ICC/IF: 1/200

Flow: 1/200 - 1/25,000

Specifications

Product Name

Grp75 Mouse mAb - Primärer Antikörper, specific to Grp75, IgG2b

Synonyme

75 kDa glucose regulated protein antibody | 75 kDa glucose-regulated protein antibody | CSA antibody | Glucose Regulated Protein antibody | Grp 75 antibody | GRP-75 antibody | GRP75 antibody | GRP75_HUMAN antibody | Heat shock 70 kDa protein 9 antibody |

Spezifikationen & Reinheit

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL

Wirtsspezies

Maus

Spezifität

Grp75

Immunogen

Recombinant protein corresponding to Human Grp75

Positive Kontrolle

WB: HeLa, HEK293, Jurkat, MCF-7, HepG2, A431, A549, K562, Mouse Brain, NIH/3T3 and C6 Whole cell Lysates Flow: HeLa cells ICC/IF: HeLa cells

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotyp

IgG2b

Lichterketten-Typ

kappa

SDS-PAGE

150.0 KDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Bilder

Western Blot: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 16.0 s 

Western Blot: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 71 kDa
Exposure time: 2.3 s 

Flow Cytometry: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Grp75 Mouse mAb (Ab181865) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

ICC/IF: Grp75 Mouse mAb (Ab181865)

Grp75 Mouse mAb (Ab181865) - ICC/IF
IF analysis of Grp75 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Grp75 Mouse mAb (Ab181865) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Anwendung

Anwendung

Dilution info

IF/ICC

1/200

WB

1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa

Flow

1/200 - 1/25,000

Zugehörige Ziele (menschlich)

HSPA9 Tchem Stress-70 protein, mitochondrial (0 Activities)

Discover Target: HSPA9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Grp75 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.