GTF2E2 Antibody - Primary antibody, specific to GTF2E2, Rabbit IgG

Cat. No.: Ab106705

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Cow, Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human GTF2E2 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Specifications

Product Name

GTF2E2 Antibody - Primary antibody, specific to GTF2E2, Rabbit IgG

Synonyms

FE antibody | General transcription factor IIE subunit 2 antibody | General transcription factor IIE, polypeptide 2, beta 34kDa antibody | gtf2e2 antibody | T2EB_HUMAN antibody | TF2E 2 antibody | TF2E2 antibody | TFIIE B antibody | TFIIE-beta antibody |

Specifications & Purity

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Host species

Rabbit

Specificity

GTF2E2

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GTF2E2.

Positive Control

WB: HEK293 and HeLa cell lysates. IHC: Human heart tissue. IF/ICC: MCF7 cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: GTF2E2 Antibody (Ab106705)

GTF2E2 Antibody (Ab106705) - Western Blot
All lanes: GTF2E2 Antibody (Ab106705) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 26, 31, 32 kDa
Exposure time: 91.2 s

IHC: GTF2E2 Antibody (Ab106705)

GTF2E2 Antibody (Ab106705) - IHC
Immunohistochemical analysis of GTF2E2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GTF2E2 Antibody (Ab106705) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: GTF2E2 Antibody (Ab106705)

GTF2E2 Antibody (Ab106705) - IF/ICC
Immunofluorescent analysis of GTF2E2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GTF2E2 Antibody (Ab106705) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Application

• Application: Dilution info
• IF/ICC:

  1/50-1/200 ;

• WB:

  1/500-1/1000 ;

• IHC:

  1/50-1/100 ;

Associated Targets(Human)

GTF2E2 Tbio Transcription initiation factor IIE subunit beta (0 Activities)

Discover Target: GTF2E2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)