Carrier Free,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Application
WB: 1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa
ICC/IF: 1/200
Flow: 1/800 - 1/200,000
Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 58 kDa
Exposure time: 15.0 s
Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4.0 s
Hsp60 Mouse mAb (Ab169190) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Hsp60 Mouse mAb (Ab169190) - ICC/IF
IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC |
1/200
|
| Flow Cytometry | 1/800 - 1/200,000
|
| WB |
1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0809905 | Certificate of Analysis | Jun 23, 2026 | Ab169190 |
| ZJ24F0809906 | Certificate of Analysis | Jun 23, 2026 | Ab169190 |
| ZJ24F0809932 | Certificate of Analysis | Jun 23, 2026 | Ab169190 |
| ZJ24F0606530 | Certificate of Analysis | Mar 18, 2026 | Ab169190 |
| ZJ24F0606531 | Certificate of Analysis | Mar 18, 2026 | Ab169190 |
| ZJ24F0606532 | Certificate of Analysis | Mar 18, 2026 | Ab169190 |
| ZJ24R0600698 | Certificate of Analysis | Jun 23, 2024 | Ab169190 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →
