KAP1 Mouse mAb - Primary antibody, specific to KAP1, IgG1

Cat. No.: Ab111687

GRADE & PURITY Validated, ExactAb™, 1.0mg/mL

Application

IF/ICC, IHC, IHC-F, IHC-P, IP, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

4E1-D12-F8

Conjugation

Unconjugated

Overview

Mouse anti Human KAP1 Antibody, 4E1-D12-F8, could be used for WB, IHC-F, IHC-P, ICC/IF, IP and so on.

Application:
WB: 1/500-1/1000
IHC-F: 1/50-1/100
IHC-P: 1/50-1/100
ICC/IF: 1/50-1/200
IP: 1/20

Protein Function
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.

Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes. Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.

Specifications

Product Name

KAP1 Mouse mAb - Primary antibody, specific to KAP1, IgG1

Synonyms

E3 SUMO protein ligase TRIM28 antibody | E3 SUMO-protein ligase TRIM28 antibody | FLJ29029 antibody | KAP 1 antibody | KAP-1 antibody | KRAB associated protein 1 antibody | KRAB interacting protein 1 antibody | KRAB-associated protein 1 antibody | KRAB-in

Specifications & Purity

ExactAb™, Validated, 1.0mg/mL

Host species

Mouse

Specificity

KAP1

Immunogen

Purified recombinant human KAP1/TIF1 beta protein fragments expressed in E.coli (AA 548-825).

Positive Control

WB: A549, Hep G2, HEK293, MCF7, HeLa, A431 lysates. IP: HeLa lysates. ICC/IF: HeLa cells. IHC: Human Spleen Tissue .

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

IgG1

Light Chain Type

kappa

SDS-PAGE

150kDa

Purification Method

Protein G purified

Concentration

1.0mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: KAP1 Mouse mAb (Ab111687)

KAP1 Mouse mAb (Ab111687) - Western Blot
All lanes: KAP1 Mouse mAb (Ab111687) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 100&75 kDa
Observed band size: 100&72 kDa

IP: KAP1 Mouse mAb (Ab111687)

KAP1 Mouse mAb (Ab111687) - IP
Immunoprecipitation analysis of KAP1 in HeLa lysates using KAP1 Mouse mAb (Ab111687).

IF: KAP1 Mouse mAb (Ab111687)

KAP1 Mouse mAb (Ab111687) - IF
IF analysis of KAP1 in HeLa using KAP1 Mouse mAb (Ab111687) .

IHC: KAP1 Mouse mAb (Ab111687)

KAP1 Mouse mAb (Ab111687) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Spleen using KAP1 Mouse mAb (Ab111687). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IP:

  1/20;

• IHC-P:

  1/50-1/100 ;

Associated Targets(Human)

TRIM28 Tbio Transcription intermediary factor 1-beta (0 Activities)

Discover Target: TRIM28

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action