Mitochondrial Malate Dehydrogenase(mMDH) Activity Assay Kit (UV Micro Method)

Cat. No.: M1515806

GRADE & PURITY BioReagent

Overview

Malate dehydrogenase (MDH, EC 1.1.1.37) is widely present in animals, plants, microorganisms, and cultured cells. In mitochondria, MDH is one of the key enzymes in the TCA cycle, catalyzing the conversion of malate to oxaloacetate. Conversely, cytoplasmic MDH catalyzes the formation of malate from oxaloacetate. Oxaloacetate is a crucial intermediate that connects multiple important metabolic pathways. Therefore, MDH plays a significant role in various cellular physiological processes, including mitochondrial energy metabolism, the malate-aspartate shuttle system, reactive oxygen species metabolism, and disease resistance. Based on coenzyme specificity, MDH is classified into NAD-dependent MDH and NADP-dependent MDH. Bacteria typically contain only NAD-MDH, while in eukaryotic cells, NAD-MDH is distributed in both the cytoplasm and mitochondria. This kit provides a simple, convenient, and rapid method for detecting mMDH activity, suitable for samples such as animal tissues, plant tissues, cells, bacteria, and serum (plasma). The principle is that mMDH catalyzes the reduction of oxaloacetate by NADH to generate malate, resulting in a decrease in light absorption at 340 nm.

Storage and Shipping

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for 6 months. Store in the dark.