Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Glycerol-3-phosphate is a key intermediate connecting glycolysis, glycerol reutilization, glycerolipid synthesis, and mitochondrial redox transfer. It can be generated by the reduction of dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase, or by the phosphorylation of glycerol ...
Nitrate reductase activity assays are used to analyze nitrate-reducing capacity in plant leaves, roots, algae, microorganisms, and soil samples. The core principle is to measure the rate at which NO₃⁻ is reduced to NO₂⁻. Common methods include in vitro enzyme extraction colorimetry, in ...
Pyruvate is a key metabolic node at the end of glycolysis. Depending on redox status, oxygen availability, cell type, and metabolic demand, pyruvate can enter different metabolic branches. PDC, LDH, and PDH respectively represent three typical routes from pyruvate toward ethanol fermentation, ...
The conversion of salicylate to catechol is a key reaction in microbial degradation of aromatic compounds. This process is usually catalyzed by salicylate hydroxylase and proceeds with the participation of flavin cofactors, reduced coenzymes, and molecular oxygen. It involves aromatic-ring ...
Intracellular electron transfer is not confined to the mitochondrial respiratory chain, but rather spans a continuous network involving glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, amino acid catabolism, oxidative folding in the endoplasmic reticulum, oxidative metabolism in ...
Diaphorase denotes a class of NAD(P)H-dependent oxidoreductase activity systems that catalyze hydride or electron transfer from NADH or NADPH to exogenous electron acceptors, thereby channeling cellular reducing equivalents to reporter molecules. Diaphorase-based reactions provide methodological ...
Coenzymes—enzymes’ indispensable partners and the behind-the-scenes drivers of biochemical reactions.
Dehydrogenases function as enzymes responsible for oxidizing and reducing carbonyl groups, particularly alcohols. These enzymes primarily rely on NAD(P)H for their activity. In the reduction process of aldehydes and ketones, baker yeast is frequently employed.
Techniques often explored alongside immunological experiments.
