GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Validated, ExactAb™, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human NECL-1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC:1/100-1/300 Protein Function： Involved in the cell-cell adhesion. Has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. Interaction with EPB41L1 may regulate structure or function of cell-cell junctions.
Specifications
Product Name
NECL-1 Antibody - Primary antibody, specific to CADM3, Rabbit IgG
A synthetic peptide derived from human CADM3 (AA 141-190)
Positive Control
WB: SK-N-SH, SH-SY5Y and NIH/3T3 cell lysates. IHC: Human tonsil tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
NECL-1 Antibody (Ab117454) - Western Blot All lanes: NECL-1 Antibody (Ab117454) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 43 kDa Observed band size: 39 kDa Exposure time: 100.0 s
NECL-1 Antibody (Ab117454) - IHC Immunohistochemical analysis of paraffin-embedded human tonsil. Tris-EDTA, pH 9.0 was used for antigen retrieval. NECL-1 Antibody (Ab117454) was diluted at 1/200 (4℃, overnight). Secondary antibody was diluted at 1/200 (room temperature, 45 min).
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/100-1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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