GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.9 mg/mL
A synthetic peptide derived from human PNPO (AA 227-261).
Positive Control
WB: Mouse brain, Rat brain, Hep G2, MCF7 and HEK293 lysates. IHC: Human gastric cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.9 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
PNPO Antibody (Ab122484) - Western Blot All lanes: PNPO Antibody (Ab122484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa Observed band size: 29 kDa Exposure time: 85.6 s
PNPO Antibody (Ab122484) - Western Blot All lanes: PNPO Antibody (Ab122484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa Observed band size: 29 kDa Exposure time: 85.6 s
PNPO Antibody (Ab122484) - IHC Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using PNPO Antibody (Ab122484). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
PNPO Antibody (Ab122484) - IF/ICC IF analysis of PNPO Antibody (Ab122484) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PNPO Antibody (Ab122484) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IF/ICC
1/200
WB
1/500-1/1000
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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