GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.0.5 mg/mL
ExactAb™, Azide Free, Validated, High Performance, 0.5 mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human Profilin 1 Antibody, clone AB01/2E11, could be used for WB, Flow Cytometry, IF/ICC and so on.
Specifications
Product Name
Profilin 1 Mouse mAb (Biotin) - Primary antibody, specific to Profilin 1
Synonyms
Actin binding protein antibody | ALS18 antibody | Epididymis tissue protein Li 184a antibody | OTTHUMP00000125244 antibody | PFN 1 antibody | Pfn antibody | PFN1 antibody | PROF1_HUMAN antibody | Profilin I antibody | Profilin-1 antibody | ProfilinI antib
Specifications & Purity
ExactAb™, Azide Free, Validated, High Performance, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androg
Host species
Mouse
Specificity
Profilin 1
Immunogen
Recombinant protein corresponding to Human Profilin 1
Positive Control
WB: HeLa, HepG2 whole cell lysates Flow Cytometry: Hela cells IF/ICC: Hela cells
Conjugation
Biotin
Grade
Azide Free, ExactAb™, High Performance, Validated
Light Chain Type
lambda
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images
Profilin 1 Mouse mAb (Biotin) (Ab220752) - IF/ICC IF analysis of Profilin 1 (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Profilin 1 Mouse mAb (Biotin) (Ab220752) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (AF647) (rp181946) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Profilin 1 Mouse mAb (Biotin) (Ab220752) - Western Blot All lanes: Profilin 1 Mouse mAb (Biotin) (Ab220752) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: SA-HRP (np156148) at 1/10000 dilution
Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 22.6 s
Profilin 1 Mouse mAb (Biotin) (Ab220752) - Flow Cytometry Flow cytometry analysis of Profilin 1 expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Profilin 1 Mouse mAb (Biotin) (Ab220752) (red) at 1/450 dilution for 30 minutes at 4°C in the dark. Then incubated with Streptavidin protein (FITC) (rp155876). Blue - Mouse IgG (Biotin) (Ab180306).
Application
Application
Dilution info
Flow Cytometry
1/100-1/1000
IF/ICC
1/100-1/1000
WB
1:1000-1:40000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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