A synthetic peptide derived from human PVRIG (AA 229-279).
Positive Control
WB: Jurkat and MOLT-4 cell lysates. IHC: Human oophoroma tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
PVRIG/CD112R Antibody (Ab123977) - Western Blot All lanes: PVRIG/CD112R Antibody (Ab123977) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 17.7 s
PVRIG/CD112R Antibody (Ab123977) - IHC Immunohistochemical analysis of paraffin-embedded human oophoroma. PVRIG/CD112R Antibody (Ab123977) was diluted at 1/200 (4°C overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
PVRIG/CD112R Antibody (Ab123977) - IF/ICC IF analysis of PVRIG/CD112R Antibody (Ab123977) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PVRIG/CD112R Antibody (Ab123977) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1:500
IHC
1/50-1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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