GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human ATG3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function： E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Specifications
Product Name
Recombinant ATG3 Antibody - Primary antibody, specific to ATG3, Rabbit IgG
Synonyms
APG3-like | Autophagy-related protein 3 | EC 2.3.2.- | hApg3 | Protein PC3-96 | 2610016C12Rik antibody | Apg 3 antibody | APG3 antibody | APG3 autophagy 3 like antibody | APG3 like antibody | APG3, S. cerevisiae, homolog of antibody | APG3-like antibody |
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Host species
Rabbit
Specificity
ATG3
Immunogen
A synthetic peptide derived from human ATG3 (AA 2-20)
Positive Control
WB: K562, HeLa, THP-1, Jurkat, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ATG3 Antibody (Ab089994) - Western Blot All lanes: Recombinant ATG3 Antibody (Ab089994) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 49.5 s
Recombinant ATG3 Antibody (Ab089994) - IF/ICC Immunocytochemistry analysis of ATG3 (green) in HeLa using Recombinant ATG3 Antibody (Ab089994) and DAPI (blue).
Recombinant ATG3 Antibody (Ab089994) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ATG3 Antibody (Ab089994). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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