GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Recombinant, Validated, ExactAb™, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human beta III Tubulin Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant beta III Tubulin Antibody - Primary antibody, specific to beta III Tubulin
Recombinant, Validated, ExactAb™, High Performance, See COA
Biochemical and Physiological Mechanisms
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Post-tra
Host species
Rabbit
Specificity
beta III Tubulin
Immunogen
A synthetic peptide derived from human beta III Tubulin (AA 400-450).
Positive Control
WB: HeLa, Hep G2, and A375 cell lysates. IHC: Human thyroid cancer tissue. IF/ICC: HeLa, A431 cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant beta III Tubulin Antibody (Ab091169) - Western Blot All lanes: Recombinant beta III Tubulin Antibody (Ab091169) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa Observed band size: 51 kDa Exposure time: 15.6 s
Recombinant beta III Tubulin Antibody (Ab091169) - Western Blot All lanes: Recombinant beta III Tubulin Antibody (Ab091169) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa Observed band size: 53 kDa Exposure time: 100.0 s
Recombinant beta III Tubulin Antibody (Ab091169) - Western Blot All lanes use Recombinant beta III Tubulin Antibody (Ab091169) at 1/10000 dilution for 1 hour at room temperature.
Recombinant beta III Tubulin Antibody (Ab091169) - IHC Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using Recombinant beta III Tubulin Antibody (Ab091169).
Recombinant beta III Tubulin Antibody (Ab091169) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant beta III Tubulin Antibody (Ab091169) at 1/50 dilution.
Recombinant beta III Tubulin Antibody (Ab091169) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant beta III Tubulin Antibody (Ab091169) at 1/50 dilution.
Recombinant beta III Tubulin Antibody (Ab091169) - IF/ICC Immunofluorescent analysis of A431 cells, using Recombinant beta III Tubulin Antibody (Ab091169) .
Application
Application
Dilution info
IHC
1/100-1/500
WB
1/1000 - 1/10000
IF/ICC
1:50-1:500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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