Recombinant F4/80 Antibody (AF647) - Primary antibody, specific to F4/80

Cat. No.: Ab213899
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2a
Clone Number
P20A44
Conjugation
AF647
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab213899-10μg
4
$197.90
25μg
Ab213899-25μg
3
$397.90
100μg
Ab213899-100μg
1
$873.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant F4/80 Antibody, clone P20A44, could be used for Flow Cytometry.

Specifications

Product Name
Recombinant F4/80 Antibody (AF647) - Primary antibody, specific to F4/80
Synonyms
ADGRE1 antibody | Adhesion G protein coupled receptor E1 antibody | Adhesion G protein-coupled receptor E1 antibody | AGRE1_HUMAN antibody | Cell surface glycoprotein EMR1 antibody | Cell surface glycoprotein F4/80 antibody | DD7A5 7 antibody | Egf like m
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Host species
Rat
Specificity
F4/80
Immunogen
Murine macrophages
Positive Control
Flow Cytometry: C57BL/6 mouse bone marrow cells
Conjugation
AF647
Excitation (nm)
Red Laser(633nm)
Emission(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Recombinant F4/80 Antibody (AF647) (Ab213899) - Flow Cytometry
Flow cytometry analysis of F4/80 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Recombinant F4/80 Antibody (AF647) (Ab213899) at 0.1 μg/test for 1 hour at 4°C. Blue – Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
ADGRE1 Tbio Adhesion G protein-coupled receptor E1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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