GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Fibrillarin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/2000 IHC: 1/50-1/100 ICC/IF: 1/50-1/100 Protein Function： S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Specifications
Product Name
Recombinant Fibrillarin Antibody - Primary antibody, specific to Fibrillarin, Rabbit IgG
A synthesized peptide derived from human Fibrillarin (AA 270-321)
Positive Control
WB: Hep G2, HEK293, U-2 OS, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Fibrillarin Antibody (Ab103454) - Western Blot All lanes: Recombinant Fibrillarin Antibody (Ab103454) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2.1 s
Recombinant Fibrillarin Antibody (Ab103454) - Western Blot All lanes: Recombinant Fibrillarin Antibody (Ab103454) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2.1 s
Recombinant Fibrillarin Antibody (Ab103454) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Fibrillarin Antibody (Ab103454).
Recombinant Fibrillarin Antibody (Ab103454) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Fibrillarin Antibody (Ab103454) at 1/50 dilution.
Recombinant Fibrillarin Antibody (Ab103454) - IHC Immunohistochemical analysis of paraffin-embedded human testis, using Recombinant Fibrillarin Antibody (Ab103454).
Application
Application
Dilution info
IHC
1/50 - 1/100
IF/ICC
1/50-1/100
WB
1/500-1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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