GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human HIF-1 alpha Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant HIF-1 alpha Antibody - Primary antibody, specific to HIF1A, Mouse IgG2b
Synonyms
ARNT interacting protein antibody | Basic helix loop helix PAS protein MOP1 antibody | bHLHe78 antibody | Class E basic helix-loop-helix protein 78 antibody | HIF 1A antibody | HIF 1alpha antibody | HIF-1alpha antibody | HIF1 A antibody | HIF1 Alpha antib
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth fa
Host species
Rabbit
Specificity
HIF1A
Immunogen
A synthetic peptide derived from human HIF-1 alpha (AA 616-674)
Positive Control
WB: Rat brain, MCF-7, C6, Ramos lysates. IHC: Human kindey tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Mouse IgG2b
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant HIF-1 alpha Antibody (Ab107478) - Western Blot All lanes: Recombinant HIF-1 alpha Antibody (Ab107478) at 1/1000 dilution Samples: Lysates at 20 µg per lane Predicted band size: 93 kDa Observed band size: 120 kDa HIF-1α is constantly synthesized but rapidly degraded under normoxic conditions, whereas reduced oxygen concentration results in stabilization of HIF-1α (PMID: 15104534).
Recombinant HIF-1 alpha Antibody (Ab107478) - Western Blot All lanes: Recombinant HIF-1 alpha Antibody (Ab107478) at 1/1000 dilution Samples: Lysates at 20 µg per lane Predicted band size: 93 kDa Observed band size: 120 kDa HIF-1α is constantly synthesized but rapidly degraded under normoxic conditions, whereas reduced oxygen concentration results in stabilization of HIF-1α (PMID: 15104534).
Recombinant HIF-1 alpha Antibody (Ab107478) - Western Blot All lanes: Recombinant HIF-1 alpha Antibody (Ab107478) at 1/1000 dilution Samples: Lysates at 20 µg per lane Predicted band size: 93 kDa Observed band size: 120 kDa HIF-1α is constantly synthesized but rapidly degraded under normoxic conditions, whereas reduced oxygen concentration results in stabilization of HIF-1α (PMID: 15104534).
Recombinant HIF-1 alpha Antibody (Ab107478) - Western Blot All lanes: Recombinant HIF-1 alpha Antibody (Ab107478) at 1/1000 dilution Samples: Lysates at 20 µg per lane Predicted band size: 93 kDa Observed band size: 120 kDa HIF-1α is constantly synthesized but rapidly degraded under normoxic conditions, whereas reduced oxygen concentration results in stabilization of HIF-1α (PMID: 15104534).
Recombinant HIF-1 alpha Antibody (Ab107478) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant HIF-1 alpha Antibody (Ab107478).
Recombinant HIF-1 alpha Antibody (Ab107478) - IHC Immunohistochemical analysis of paraffin-embedded human kindey, using Recombinant HIF-1 alpha Antibody (Ab107478).
Application
Application
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
IHC
1/100-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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