GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
E. coli
Accession #
P01584
Protein Tag
No tag
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
1. Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL. 2. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 52.68 ng/mL. 3. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC50 of 22.81 ng/mL.
Carrier Free,Bioactive,ActiBioPure™,PBS Only,≥95%(SDS-PAGE) ActiBioPure™,Bioactive,Carrier Free,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Protein Function: IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in humans. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
1. Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL. 2. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 52.68 ng/mL. 3. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC50 of 22.81 ng/mL.
15.0 kDa, under reducing conditions; 15.1 kDa, under non-reducing conditions
Molecule Type
Protein
Storage and Shipping
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
Images
Recombinant Human IL-1β Protein (rp156012) - ELISA Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC₅₀ of 52.68 ng/mL.
Recombinant Human IL-1β Protein (rp156012) - ELISA Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC₅₀ of 22.81 ng/mL.
Recombinant Human IL-1β Protein (rp156012) - SDS-PAGE 3 μg/lane of Recombinant Human IL-1β Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.0 kDa under reducing conditions and 15.1 kDa under non-reducing conditions.
Recombinant Human IL-1β Protein (rp156012) - Binding Activity Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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