Recombinant Human S100B Protein, >95%(SDS-PAGE)

Cat. No.: rp156671

GRADE & PURITY Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Expression System

E. coli

Accession #

P04271

Protein Tag

N-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Overview

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. 

Description： 

100B, previously called S100 beta, belongs to the S100 family within the EF-hand superfamily of Ca2+ binding proteins. S100 proteins contain two EF-hand motifs that differ in affinity, separated by a hinge region with a hydrophobic cleft that is exposed upon Ca2+ binding. S100B is a 91 amino acid (aa) protein, after removal of the initial methionine, and is found as homodimers of 10.4 kDa monomers. Human S100B shares 99%, 98%, 100%, 99% and 97% aa sequence identity with mouse, rat, rabbit, equine and bovine S100B, respectively. Within the S100 family, human S100B shows the highest aa identity (59%) with S100A1. S100B is expressed primarily by astrocytes and oligodendrocytes in the central nervous system, and by Schwann cells in the peripheral nervous system. Ca2+-bound S100B interacts in vitro with at least 20 cytoplasmic proteins, including several structural molecules such as tubulin and GFAP. It can inhibit the phosphorylation of these kinase substrates and others such as tau and neuromodulin. Astrocytes can secrete S100B, which then acts in a cytokine-like manner. Nanomolar concentrations of S100B are secreted constitutively, promote proliferation, and are neurotrophic and anti-apoptotic. Blood levels of S100B reflect extracellular concentrations within the nervous system, and are elevated in Down’s syndrome, Alzheimer’s disease and Tourette’s syndrome, metabolic stress, acute brain injury and brain tumors. Micromolar concentrations of S100B can be destructive and pro-apoptotic; they induce the expression of iNOS, COX-2, IL-1, IL‑6 and TNF-alpha by microglia, astrocytes or neurons. Most extracellular actions of S100B can be mediated by RAGE (receptor for advanced glycation end products), which is also a receptor for other S100 proteins.

Specifications

Product Name

Recombinant Human S100B Protein, >95%(SDS-PAGE)

Synonyms

beta (neural) | NEF | S100 beta | S100 calcium binding protein B | S100 calcium-binding protein B | S100 calcium-binding protein, beta (neural) | S-100 calcium-binding protein, beta chain, 10protein S100-B | S-100 protein beta chain | S-100 protein subuni

Grade

ActiBioPure™, Azide Free, Bioactive, Carrier Free, His Tag, PBS Only

Specifications & Purity

Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Purity

>95%(SDS-PAGE)

Bioactivity

Testing in progress

Endotoxin Concentration

<1.0 EU/μg

Expression System

E. coli

Amino Acids

1-92 aa

Sequence

MGHHHHHHMSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDNDGDGECDFQEFMAFVAMVTTACHEFFEHE

Protein Tag

N-His

Accession #

P04271

Predicted molecular weight

11.7 kDa

SDS-PAGE

10.6 kDa, reducing conditions; 10.6 kDa, non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze/thaw cycle.

Images

SDS-PAGE: Recombinant Human S100B protein (rp156671)

Recombinant Human S100B protein (rp156671) - SDS-PAGE
Recombinant Human S100B protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.6 kDa.