Recombinant Human TIM 3 Protein, ＞95% (SDS-PAGE)

Cat. No.: rp152388

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, Fc tag, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

Q8TDQ0

Protein Tag

C-hFc & His

Expression system

HEK293

Endotoxin Concentration

<0.1 EU/μg

Bioactivity

Immobilized Human Galectin-9 at 2 μg/mL can bind recombinant human TIM-3 with a linear range of 0.3 - 5 μg/mL.

Overview

Purity
>95% SDS-PAGE.

Function
Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock.

Specifications

Product Name

Recombinant Human TIM 3 Protein, ＞95% (SDS-PAGE)

Synonyms

CD366 | FLJ14428 | HAVCR2 | HAVcr-2 | hepatitis A virus cellular receptor 2 | kidney injury molecule-3 | KIM-3 | SPTCL | T cell immunoglobulin mucin 3 | T cell immunoglobulin mucin-3 | T-cell immunoglobulin and mucin domain-containing protein 3 | TIM 3 |

Grade

ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, Fc tag, His Tag

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, Fc tag, ≥95%(SDS-PAGE)

Purity

＞95% (SDS-PAGE)

Bioactivity

Immobilized Human Galectin-9 at 2 μg/mL can bind recombinant human TIM-3 with a linear range of 0.3 - 5 μg/mL.

Endotoxin Concentration

<0.1 EU/μg

Expression System

HEK293

Species

Human

Amino Acids

22-200 aa

Sequence

SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRENLYFQGMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH

Protein Tag

C-Fc & His

Accession #

Q8TDQ0

Predicted molecular weight

49 kDa

SDS-PAGE

60-80 kDa, under reducing conditions.

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

Reconstitute in sterile H2O to a concentration of 0.1-0.5 mg/ml.

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at﹣20~ ﹣80℃ for more than 1 year.

Images

Protein Bioactivity: Recombinant Human TIM 3 Protein (rp152388)

Recombinant Human TIM 3 Protein (rp152388) - Protein Bioactivity
Immobilized Human Galectin-9 at 2 μg/mL can bind recombinant human TIM-3 (rp152388) with a linear range of 0.3 - 5.0 μg/mL.

SDS-PAGE: Recombinant Human TIM 3 Protein (rp152388)

Recombinant Human TIM 3 Protein (rp152388) - SDS-PAGE
3 μg/lane of Recombinant Human TIM 3 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 60 - 80 kDa.