Recombinant L-Lactate Dehydrogenase (L-LDH), CAS No.9001-60-9

CAS: 9001-60-9 Cat. No.: L1492991 EC Number: 232-617-8 PubChem CID: 135297138

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, >260 U/mg protein

Accession #

P00339,P00336,Q9TSX5

Bioactivity

>260 U/mg protein

Overview

L-Lactate Dehydrogenase (L-LDH) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD⁺ and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another.

Reaction Equation

 

Specific Activity

U/mg protein: > 260 units
Contaminants
Malate dehydrogenase: < 0.03%
Myokinase: < 0.01%
Pyruvate kinase: < 0.003%
Glutamic-pyruvic transaminase*: < 0.03%
Glutamic-oxaloacetic transaminase*: < 0.03%
*Including α-Hydroxyglutarate dehydrogenase activity

Properties

pH stability: pH 5.5 - 8.0 (25°C, 1 week)
Thermal stability: ≤55°C (pH 7.5, 10 min)
Optimum pH: 7.0 - 8.0
Optimum temp.: 70°C
Km value: 6.5 × 10⁻⁵ mol/L (Pyruvate); 1.8 × 10⁻⁵ mol/L (NADH)
Molecular weight: 36 kDa (SDS - PAGE)

Assay Procedure

I Spectrophotometric Method

Wavelength 340 nm, Light path length 1 cm
Final volume 3.17 mL, Temperature 25
Pipette the following reagents into a cuvette

3.00 mL	K-phosphate buffer (0.1 mol/L, pH 7.0)
0.10 mL	Na-pyruvate (25.4 mmol/L)
0.05 mL	NADH (10 mg/mL) dissolved in Tris (10 mmol/L)
0.02 mL	rLDH (PH) (approx. 3 U/mL)

II Calculation

A/min: The change in absorbance at 340 nm/minute
V: Total volume of reaction mixture (3.17 mL)
D: Enzyme dilution factor
6.3=mmol/L extinction coefficient of NADH (L⋅mmol⁻¹⋅cm⁻¹) 
d=Light path length (1cm)
v=Volume of enzyme sample (0.02mL)

Reference Data

Specifications

Product Name

Recombinant L-Lactate Dehydrogenase (L-LDH), CAS No.9001-60-9

Synonyms

L-lactate dehydrogenase | L-lactic acid dehydrogenase | L-lactic dehydrogenase | LDHA | LDHB | LDHC | LDH-A | LDH-M | LDH-B | LDH-H | LDH-C | LDH-X

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

ActiBioPure™, Bioactive, High Performance, EnzymoPure™, Recombinant, >260 U/mg protein

Biochemical and Physiological Mechanisms

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD⁺.

Bioactivity

>260 U/mg protein

Accession #

P00339, P00336, Q9TSX5

CAS

9001-60-9

Enzyme Commission Number

1.1.1.27

Molecule Type

Enzyme

Storage and Shipping

Concentration

>260 U/mg protein

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

References

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