GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Melanoma gp100 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/50-1/200 Protein Function： Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Specifications
Product Name
Recombinant Melanoma gp100 Antibody - Primary antibody, specific to Melanoma gp100, Rabbit IgG
A synthetic peptide derived from human Melanoma gp100 (AA 600-661).
Positive Control
WB: Human melanoma lysates. IHC: Human melanoma tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Melanoma gp100 Antibody (Ab114936) - Western Blot Western blot analysis of Recombinant Melanoma gp100 Antibody (Ab114936) expression in human melanoma lysate.
Predicted band size: 70 kDa Observed band size: 100 & 26 kDa The gp100 protein exhibits different cleavage forms in melanoma cells. In one study (PMID: 15096515), gp100 was detected in the melanoma cell line MNT-1 as two major specific bands, measuring 100 kDa and 26 kDa.
Recombinant Melanoma gp100 Antibody (Ab114936) - IHC Immunohistochemical analysis of paraffin-embedded human melanoma, using Recombinant Melanoma gp100 Antibody (Ab114936).
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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