GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human NCAM1 Antibody, Recombinant, could be used for WB, IF/ICC and so on.
Specifications
Product Name
Recombinant NCAM1 Antibody - Primary antibody, specific to NCAM1, Rabbit IgG
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Host species
Rabbit
Specificity
NCAM1
Immunogen
A synthetic peptide derived from human NCAM1 (AA 801-858).
Positive Control
WB: SH-SY5Y lysates. IF/ICC: HeLa, SH-SY5Y cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NCAM1 Antibody (Ab117182) - Western Blot All lanes: Recombinant NCAM1 Antibody (Ab117182) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 95 kDa Observed band size: 140, 213 kDa This antibody recognizes isoforms NCAM140 and NCAM180, at 140 and 180 kDa respectively. NCAM contains a number of potential glycosylation sites which may explain its migration at a higher molecular weight than predicted. This banding pattern has been observed in the literature PMID:21389209, 9696812. Exposure time: 59.5 s
Recombinant NCAM1 Antibody (Ab117182) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant NCAM1 Antibody (Ab117182) at 1/50 dilution.
Recombinant NCAM1 Antibody (Ab117182) - IF/ICC Immunofluorescent analysis of SH-SY5Y cells, using Recombinant NCAM1 Antibody (Ab117182) at 1/50 dilution.
Recombinant NCAM1 Antibody (Ab117182) - IF/ICC Immunofluorescent analysis of SH-SY5Y cells, using Recombinant NCAM1 Antibody (Ab117182) at 1/50 dilution.
Application
Application
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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