Recombinant Rat Prolactin Protein, ＞98%(SDS-PAGE)

Cat. No.: rp155196

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, PBS Only, ≥98%(SDS-PAGE)

Expression System

E.coli

Accession #

P01237

Protein Tag

No tag

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

Overview

Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.
Background
The neuroendocrine pituitary hormone Prolactin (PRL), also known as lactotrophin, mamotrophin, luteotropic hormone (LTH), or luteotropin, is a secreted hormone that affects reproduction and homeostasis in vertebrates. The functions of PRL can be placed in six broad categories: 1) reproduction and lactation; 2) growth and development; 3) endocrinology and metabolism; 4) brain and behavior; 5) immunomodulation; and 6) electrolyte balance. PRL is secreted by the anterior pituitary gland, mammary gland, placenta, brain, uterus, decidua, dermal fibroblasts, B cells, T cells, NK cells, and some breast cancer cell lines. Although the major form of PRL is a 23 kDa monomeric protein, splice variants of 14, 16, and 22 kDa have been identified. PRL has also been found to be glycosylated, phosphorylated, dimerized, and polymerized. Glycosylation, phosphorylation, dimerization, or polymerization of PRL result in lower activity.
Cell activation by PRL is mediated by a single chain membrane-bound protein belonging to the class 1 cytokine superfamily. The PRL receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced. Although the cytoplasmic domains of the different isoforms vary in length and composition, their extracellular domains are identical. In rats, three major PRL receptor isoforms have been described, a short (291 amino acid), an intermediate (393 amino acid), and a long (591 amino acid). PRL receptors are found in mammary tissue, pituitary gland, brain, heart, lung thymus, spleen, liver, pancreas, kidney, adrenal gland, uterus, skeletal muscle, and skin. A soluble form of PRL-R containing the 206NH2-terminal amino acids of the extracellular domain is secreted by mammary epithelial cells and is found in milk. Binding of the transmembrane PRL R results in ligand dimerization followed by binding and phosphorylation of Jak2. Jak2 then phosphorylates STAT and the long form of PRL R. C-src, fyn, and the Ras/Raf/MAP kinase pathway have also been found to be activated upon PRL R ligand binding.

Specifications

Product Name

Recombinant Rat Prolactin Protein, ＞98%(SDS-PAGE)

Synonyms

Decidual prolactin | GHA1 | Growth hormone A1 | Lactogenic hormone | Luteotropic hormone | Mammotropin | PRL | Prolactin | Prolactin precursor

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, PBS Only

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, PBS Only, ≥98%(SDS-PAGE)

Purity

＞98%(SDS-PAGE)

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

Endotoxin Concentration

<1.0 EU/μg

Expression System

E.coli

Species

Rat

Amino Acids

30-226 aa

Sequence

LPVCSGGDCQ TPLPELFDRV VMLSHYIHTL YTDMFIEFDK QYVQDREFIA KAINDCPTSS LATPEDKEQA QKVPPEVLLN LILSLVHSWN DPLFQLITGL GGIHEAPDAI ISRAKEIEEQ NKRLLEGIEK IISQAYPEAK GNEIYLVWSQ LPSLQGVDEE SKDLAFYNNI RCLRRDSHKV DNYLKFLRCQ IVHKNNC

Protein Tag

No tag

Accession #

P01237

Predicted molecular weight

22.6 kDa

SDS-PAGE

22.6 kDa

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein ali

Images

Protein Bioactivity: Recombinant Rat Prolactin Protein (rp155196)

Recombinant Rat Prolactin Protein (rp155196)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

SDS-PAGE: Recombinant Rat Prolactin Protein (rp155196)

Recombinant Rat Prolactin Protein (rp155196)-SDS-PAGE
Recombinant Rat Prolactin Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 22.6 kDa.