GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.5 mg/mL
ExactAb™, Carrier Free, Validated, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Mouse anti Human S100 alpha 2/S100A2 Antibody, Monoclonal (F92S417), could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
S100 alpha 2/S100A2 Mouse mAb - Primärer Antikörper, specific to S100A2
Synonyme
CAN19 antibody | MGC111539 antibody | Protein S 100L antibody | Protein S-100L antibody | Protein S100 A2 antibody | Protein S100-A2 antibody | Protein S100Lantibody | S100 A2 antibody | S100 calcium binding protein A2 antibody | S100 calcium-binding prot
Spezifikationen & Reinheit
ExactAb™, Carrier Free, Validated, 0.5 mg/mL
Biochemische und physiologische Mechanismen
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in
Wirtsspezies
Maus
Spezifität
S100A2
Immunogen
Recombinant protein of human S100 alpha 2/S100A2.
Positive Kontrolle
WB: A431 cell lysate. IHC: Human skin tissue. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
Carrier Free, ExactAb™, Validated
Lichterketten-Typ
kappa
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein G purified
Konzentration
0.5 mg/mL
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
S100 alpha 2/S100A2 Mouse mAb (Ab126175) - Western Blot All lanes: S100 alpha 2/S100A2 Mouse mAb (Ab126175) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouose IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 85.8 s
S100 alpha 2/S100A2 Mouse mAb (Ab126175) - IHC Immunohistochemical analysis of S100-A2 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with S100 alpha 2/S100A2 Mouse mAb (Ab126175) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
S100 alpha 2/S100A2 Mouse mAb (Ab126175) - IF/ICC Immunofluorescent analysis of S100-A2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with S100 alpha 2/S100A2 Mouse mAb (Ab126175) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Anwendung
Anwendung
Dilution info
WB
1/500-1/2000
IHC
1/50-1/200
IF/ICC
1/10 - 1/50
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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