GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
Application
Capture Antibody, CLIA, Detection Antibody, ELISA, FIA, GICA, LF
25.6 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 174.5 kDa, under non-reducing conditions.
Verfahren zur Reinigung
Affinity chromatography
Übereinstimmende Paare
[Ab008408] Monoclonal S100B Antibody as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
Konzentration
See COA
Lagerung und Versand
Form
Purified polyclonal antibodies Antibody
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 37°C short term（7 days）Store at 4°C short term（30 days）. Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Sandwich ELISA - S100B protein standard curve S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Sensitivity: 30.3 pg/mL Range: 0.1 ng/mL - 3.2 ng/mL
Sandwich ELISA - S100B protein standard curve S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Sensitivity: 18.8 pg/mL Range: 0.13 ng/mL - 3.1 ng/mL
S100B Mouse mAb (Ab008407) - SDS-PAGE 3 μg/lane of S100B Mouse mAb (Ab008407) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 26.0 kDa (Light Chain) & 54.2 kDa (Heavy Chain) under reducing conditions and 178.7 kDa under non-reducing conditions.
Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA ·Capture antibody: S100B Mouse mAb (Ab008408) ·Detection antibody: S100B Mouse mAb (Ab008407)
Anwendung
Anwendung
Dilution info
CLIA
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
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