SDS-PAGE Stacking Gel Master Mix

Cat. No.: S743297

Overview

Aladdin's SDS-PAGE Stacking Gel Master Mix contains almost all reagents required for the preparation of SDS-PAGE stacking gels, except for polymerization catalysts such as ammonium persulfate and TEMED.This product can be used to prepare the most commonly used 5% stacking gels. If other concentrations are desired, we recommend purchasing Acr-Bis (29

Precautions：

Ammonium persulfate or its substitute (, ST005) and TEMED are required but not provided in this product.The stacking gel must be prepared after the resolving gel is polymerized.This product contains Acr-Bis which is potentially neurotoxic. Please take effective measures to avoid direct contact with the human body or inhalation.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.According to the size of a target protein, decide an appropriate concentration of SDS-PAGE resolving gel. Please refer to the table below.SDS-PAGE Resolving Gel Concentration Optimal Separation Range6%50-150kD8%30-90kD10 -80kD12%12-60kD15%10-40kD2.Prepare a 10% ammonium persulfate solution with ddH2O or other high-purity water. Solution of ammonium persulfate or its substitute is prone to failure and should be prepared freshly or kept frozen for multiple uses.3.After the resolving gel is fully polymerized, prepare the stacking gel according to the table below. For example, add 30μl of 10% ammonium persulfate and 3μl of TEMED into 3ml of SDS-PAGE Stacking Gel Master Mix, mix well, and immediately pour onto the top of the resolving gel without sealing liquid. Insert the comb and leave at room temperature (~25℃) until fully solidified (usually within 10-30 minutes).Note: The amount of polymerization catalyst in the table below is recommended for polymerization at 25℃. It can be adjusted appropriately according to the temperature. For example, when the room temperature is lower than 20℃, add more TEMED and 10% ammonium persulfate appropriately to promote gel solidification. ReagentsVolume of each component (ml) required for different volumes of SDS-PAGE resolving gelSDS-PAGE Stacking Gel Master Mix234681010% ammonium persulfate or its substitute0.020.030.040.060.080.1TEMED 0.0020.0040.0060.0080.0120.024.If the prepared gel is not to be used on the same day, it can be stored at 4℃ for 1-2 days.

Specifications

Product Name

SDS-PAGE Stacking Gel Master Mix

Molecule Type

Bioreagents/Buffer

Storage and Shipping

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Stability And Storage

Store at 4℃ in the dark for up to 1 year.

Precautions：

TMB is irritating. Please take effective measures to avoid inhalation or direct contact with skin.