GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Validated, Carrier Free, ExactAb™, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human SERPINB1/PI2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.
Specifications
Product Name
SERPINB1/PI2 Antibody - Primary antibody, specific to SERPINB1/PI2, Rabbit IgG
Synonyms
Anti elastaseantibody | EI antibody | ELANH2antibody | Epididymis luminal protein 57 antibody | HEL57 antibody | ILEU_HUMAN antibody | LEI antibody | Leukocyte elastase inhibitor antibody | M/NEI antibody | MNEIantibody | Monocyte/neutrophil elastase inhi
Specifications & Purity
Validated, Carrier Free, ExactAb™, 1.0 mg/mL
Biochemical and Physiological Mechanisms
Protein Function：
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
Host species
Rabbit
Specificity
SERPINB1/PI2
Immunogen
Recombinant full protein of human SERPINB1/PI2.
Positive Control
WB: Mouse spleen, THP-1 and U937 lysates. IHC: Human liver cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
SERPINB1/PI2 Antibody (Ab127151) - Western Blot All lanes: SERPINB1/PI2 Antibody (Ab127151) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 42 kDa Observed band size: 37, 41 kDa Exposure time: 7.3 s
SERPINB1/PI2 Antibody (Ab127151) - Western Blot All lanes: SERPINB1/PI2 Antibody (Ab127151) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 26.3 s
SERPINB1/PI2 Antibody (Ab127151) - IHC Immunohistochemical analysis of SerpinB1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with SERPINB1/PI2 Antibody (Ab127151) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
SERPINB1/PI2 Antibody (Ab127151) - IF/ICC Immunofluorescent analysis of SERPINB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with SERPINB1/PI2 Antibody (Ab127151) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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