Smad3 Mouse mAb - Primärer Antikörper, specific to Smad3, Mouse IgG2b

Cat. No.: Ab186978

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry, WB

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG2b

Clone Number

FG02/4F11

Conjugation

Unconjugated

Übersicht

Application
WB: 1/1000 – 1/100,000
Flow: 1/200 - 1/2,000

Specifications

Product Name

Smad3 Mouse mAb - Primärer Antikörper, specific to Smad3, Mouse IgG2b

Synonyme

DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca

Spezifikationen & Reinheit

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Wirtsspezies

Maus

Spezifität

Smad3

Immunogen

Recombinant full length protein corresponding to Human Smad3

Positive Kontrolle

WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotyp

Mouse IgG2b

Lichterketten-Typ

kappa

SDS-PAGE

150.0 KDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Bilder

Western Blot: Samd3 Mouse mAb (Ab186978)

Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 1.1 s 

Western Blot: Samd3 Mouse mAb (Ab186978)

Samd3 Mouse mAb (Ab186978) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab186978) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 47 kDa
Exposure time: 69.1 s 

Flow Cytometry: Samd3 Mouse mAb (Ab186978)

Samd3 Mouse mAb (Ab186978) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  1/200-1/2000;

• WB:

  1/1000 – 1/100,000

Zugehörige Ziele (menschlich)

SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)

Discover Target: SMAD3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)

Discover Target: SMAD3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)

Discover Target: SMAD3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Smad3 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.