Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b

Cat. No.: Ab188096
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Flow Cytometry, IF/ICC, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG2b
Clone Number
FG01/2C1
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab188096-10μl
≥10
$79.90
50μl
Ab188096-50μl
7
$259.90
100μl
Ab188096-100μl
2
$419.90
1ml
Ab188096-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$2,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

WB: 1/1000 – 1/100,000

Flow: 1/200 - 1/2,000

ICC/IF: 1/200

Specifications

Product Name
Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b
Synonyms
DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
Host species
Mouse
Specificity
Smad3
Immunogen
Recombinant full length protein corresponding to Human Smad3
Positive Control
WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells ICC/IF: U-2 OS cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
Mouse IgG2b
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 49 kDa
Exposure time: 0.9 s 

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 29.6 s 

Samd3 Mouse mAb (Ab188096) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Samd3 Mouse mAb (Ab188096) - ICC/IF
IF analysis of Samd3 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Samd3 Mouse mAb (Ab188096) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application
ApplicationDilution info
Flow Cytometry

1/200-1/2000

IF/ICC

1/200

WB

1/1000 – 1/100,000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607491Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607492Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607493Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24R0600677Certificate of AnalysisJun 14, 2024 Ab188096
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

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