5-Carboxyfluorescein diacetate(5-CFDA) - ≥95% , CAS No.79955-27-4

CAS: 79955-27-4 Cat. No.: C114919 Molecular Weight: 460.39 Beilstein Registry Number: 68265 EC Number: 635-966-8

GRADE & PURITY ≥95%

Synonyms

5-CFDA | HY-D0047 | PD063611 | Cfda-5 | C2859 | 3',6'-Bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-5-carboxylic acid | 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid | WPUZGNPQMIWOHE-UHFFFAOYSA-N | 3',6'-bis(a

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Specifications

Synonyms

5-CFDA | HY-D0047 | PD063611 | Cfda-5 | C2859 | 3', 6'-Bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H), 9'-(9H)xanthene)-5-carboxylic acid | 3', 6'-diacetyloxy-3-oxospiro[2-benzofuran-1, 9'-xanthene]-5-carboxylic acid | WPUZGNPQMIWOHE-UHFFFAOYSA-N | 3', 6'-bis(a

Specifications & Purity

≥95%

Biochemical and Physiological Mechanisms

Staining of cells requires 5-carboxyfluorescein diacetate to differentiate between live and dead cells. Helps monitor cell membrane integrity and integrity. 5-Carboxyfluorescein diacetate is also used to measure intracellular pH. 5-Carboxyfluorescein diac

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥95%

Names and Identifiers

• Pubchem Sid: 488187816
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488187816
• Canonical Smiles: CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C
• IUPAC Name: 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• InChIKey: WPUZGNPQMIWOHE-UHFFFAOYSA-N
• INCHI: 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
• Isomeric SMILES: CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C
• WGK Germany: 3
• Molecular Weight: 460.39
• Beilstein: 68265
• Reaxy-Rn: 68265
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=68265&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Tetracarboxylic acids and derivatives M-phthalic acid and derivatives Diarylethers Phthalides Benzofuranones Isobenzofurans Lactones Carboxylic acid esters Oxacyclic compounds Carboxylic acids Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Tetracarboxylic acid or derivatives - Meta_phthalic_acid - Diaryl ether - Benzofuranone - Isobenzofuranone - Phthalide - Isocoumaran - Isobenzofuran - Benzenoid - Carboxylic acid ester - Lactone - Carboxylic acid derivative - Carboxylic acid - Ether - Oxacycle - Organic oxygen compound - Organic oxide - Hydrocarbon derivative - Carbonyl group - Organooxygen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

ABCC2 Tchem Canalicular multispecific organic anion transporter 1 (1191 Activities)

Discover Target: ABCC2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SLCO1B1 Tchem Solute carrier organic anion transporter family member 1B1 (2672 Activities)

Discover Target: SLCO1B1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SLCO1B3 Tchem Solute carrier organic anion transporter family member 1B3 (2517 Activities)

Discover Target: SLCO1B3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Solubility: chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear
• Sensitivity: Heat Sensitive
• Molecular Weight: 460.400 g/mol
• XLogP3: 3.200
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 9
• Rotatable Bond Count: 5
• Exact Mass: 460.079 Da
• Monoisotopic Mass: 460.079 Da
• Topological Polar Surface Area: 125.000 Ų
• Heavy Atom Count: 34
• Formal Charge: 0
• Complexity: 828.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Xiao-min Li, Lie-hua Che, Ying Wu, Cong Li, Bao-cai Xu.  (2024)  An effective strategy for improving the freeze-drying survival rate of Lactobacillus curvatus and its potential protective mechanism.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.103794]